Directed evolution on the cold adapted properties of TAB5 alkaline phosphatase
نویسندگان
چکیده
منابع مشابه
Directed evolution on the cold adapted properties of TAB5 alkaline phosphatase.
Psychrophilic alkaline phosphatase (AP) from the Antarctic strain TAB5 was subjected to directed evolution in order to identify the key residues steering the enzyme's cold-adapted activity and stability. A round of random mutagenesis and further recombination yielded three thermostable and six thermolabile variants of the TAB5 AP. All of the isolated variants were characterised by their residua...
متن کاملCrystal structure of alkaline phosphatase from the Antarctic bacterium TAB5.
Alkaline phosphatases (APs) are non-specific phosphohydrolases that are widely used in molecular biology and diagnostics. We describe the structure of the cold active alkaline phosphatase from the Antarctic bacterium TAB5 (TAP). The fold and the active site geometry are conserved with the other AP structures, where the monomer has a large central beta-sheet enclosed by alpha-helices. The dimer ...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 2008
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/gzn009